top of page
AdobeStock_179309453.jpeg

Smart DNA Synthesis Technology

The Variants You Need,
at 1/10th of the Price.

DNA Service Providers, Antibody, Protein and Enzyme Engineers all need Homology Path

Technology

Our Technology

From affinity maturation to protein engineering, variants will always be needed to find the required activity. Ninthbio's Homology Path DNA synthesis tools design your DNA populations to be assembled from the least number of oligos possible. Our approach is so aggressive, it typically saves 90% when compared to synthesis by a service provider. We design the oligos to assemble with a simple PCR reaction; any molecular biology lab can take control of their DNA synthesis pipeline.

Learn about Protein Evolution, Inc.'s success with Homology Path in the video below.

NinthBioDNAStrandBGGreen.jpg
Software

OUR SOFTWARE

Homology Path

Homology Path designs the oligos needed to synthesize a population of DNA sequences by finding the shared scaffolds and designing the build around those common areas.

HOW IT WORKS

1

Import FASTA File

Import the sequences of interest as a single FASTA file.

2

Check Complexity

Our complexity analysis shows you where the potential sequence issues are, so you can optimize to best meet your synthesis and expression requirements

3

Generate Population Design

Run the oligo designer, it will automatically find the possible recycling and build the most economical design

4

Oligo Order Is Generated

The list of oligos needed are generated in a format that can be submitted directly to your oligo vendor for production

5

Pool the Oligos

Pooling instructions are generated for you to pool the oligos by hand or by automation; we accommodate all automation formats

6

Run PCR

Run standard PCR reactions on the oligo pools just as you would a DNA template

Interested in a free trial?

Hardware

DNA Assembly

Overview

Our pooling instructions and assembly are robust and ideal for automation platforms. Our customers have used almost every imaginable transfer method from manual pipetting for small projects, or using the Labcyte Echo for pooling thousands of reactions per day

 

Oligo Pooling

The instructions generated from Homology Path can be uploaded directly to most automation systems for the pooling of your oligos

 

PCR Assembly

The oligos pools are designed to assemble in a high fidelity robust reaction. This assembly reaction is perfectly suited for automated Thermal Cyclers; both stand-alone, or integrated with your automation

Request Assembly Protocol

Contact

Get in Touch

Thanks for submitting!

bottom of page